The cognate methyltransferase methylates the same specific sequence that its paired endonuclease recognizes and renders the modified DNA resistant to cleavage by the endonuclease so that the host DNA can be properly protected. De Bruijin, et al., Chapman & Hall, New York, 78-92 (1998)), in which they form part of restriction-modification systems, which mainly consist of an endonuclease and a methyltransferase. They are ubiquitously present among prokaryotic organisms ( Raleigh, et al., Bacterial Genomes Physical Structure and Analysis, Ch.8, eds. Pingoud, Springer-Verlag Berlin Heidelberg, New York, 393-407 (2004)). Restriction endonucleases are enzymes that cleave doublestranded DNAs in a sequence-specific manner ( Roberts, R.J., Proc Natl Acad Sci U S A, 102:5905-5908 (2005) Roberts, et al., Nucleic Acids Res, 31:1805-1812 (2003) Roberts, et al., Nucleic Acids Res, 33: D230-232 (2005) Alves, et al., Restriction Endonucleases, " Protein Engineering of Restriction Enzymes," ed. C12Q1/44- Measuring or testing processes involving enzymes, nucleic acids or microorganisms Compositions therefor Processes of preparing such compositions involving hydrolase involving esterase.C12Q1/34- Measuring or testing processes involving enzymes, nucleic acids or microorganisms Compositions therefor Processes of preparing such compositions involving hydrolase.C12Q1/00- Measuring or testing processes involving enzymes, nucleic acids or microorganisms Compositions therefor Processes of preparing such compositions.C12Q- MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS COMPOSITIONS OR TEST PAPERS THEREFOR PROCESSES OF PREPARING SUCH COMPOSITIONS CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES.C12N15/1086- Preparation or screening of expression libraries, e.g.C12N9/16- Hydrolases (3) acting on ester bonds (3.1).C12N9/00- Enzymes Proenzymes Compositions thereof Processes for preparing, activating, inhibiting, separating or purifying enzymes.evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms C12N15/1058- Directional evolution of libraries, e.g.C12N15/1034- Isolating an individual clone by screening libraries.C12N15/10- Processes for the isolation, preparation or purification of DNA or RNA.plasmids, or their isolation, preparation or purification Use of hosts therefor C12N15/00- Mutation or genetic engineering DNA or RNA concerning genetic engineering, vectors, e.g.C12N- MICROORGANISMS OR ENZYMES COMPOSITIONS THEREOF PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS MUTATION OR GENETIC ENGINEERING CULTURE MEDIA.C12- BIOCHEMISTRY BEER SPIRITS WINE VINEGAR MICROBIOLOGY ENZYMOLOGY MUTATION OR GENETIC ENGINEERING.Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by New England Biolabs Inc filed Critical New England Biolabs Inc Publication of EP2395079A1 publication Critical patent/EP2395079A1/en Application granted granted Critical Publication of EP2395079B1 publication Critical patent/EP2395079B1/en Status Active legal-status Critical Current Anticipated expiration legal-status Critical Links Original Assignee New England Biolabs Inc Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) ( en Inventor Zhenyu Zhu Aine Blanchard Shuang-Yong Xu Shengxi Guan Hua Wei Penghua Zhang Dapeng Sun Siu-Hong Chan Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Active Application number EP11075112.0A Other languages German ( de) Google Patents EP2395079B1 - Screening methodÄownload PDF Info Publication number EP2395079B1 EP2395079B1 EP11075112.0A EP11075112A EP2395079B1 EP 2395079 B1 EP2395079 B1 EP 2395079B1 EP 11075112 A EP11075112 A EP 11075112A EP 2395079 B1 EP2395079 B1 EP 2395079B1 Authority EP European Patent Office Prior art keywords activity buffer dna neb4 cleavage Prior art date Legal status (The legal status is an assumption and is not a legal conclusion.
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